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Objective:To investigate the regulation of proliferation and differentiation of bone marrow mesenchymal stem cells(BM-SCs)by CKIP-1 in vitro.Methods:BMSCs from CKIP-1 nock out(KO)and wild type(WT)C57 mice were isolated and cultured u-sing adherence method in vitro.BMSCs of the 3rd passage were induced to osteogenic and adipgenic differentiation.Cell proliferation was examined by MTT assay.Cell surface markers were tested by FCM.The osteogenic and adipogenic differentiation was studied by alkaline phosphatase (ALP)staining,alizarin red staining and oil red O staining.Results:The proliferation and cell marke expression of the 2 groups were similar.ALP staining of KO group was strong than that of WT group after osteogenic induction.Alizarin red stai-ning showed that there were more mineralized nodules in BMSCs of KO group than in those of WT group.Oil red O staining of KO mice BMSCs was stronger than that of WT.Conclusion:CKIP-1 deficiency can enhance the osteogenic and adipogenic differentiation without influence on the proliferation of BMSCs.
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<p><b>OBJECTIVE</b>This study aims to compare the changes of hyoid bone position before and after treatment of Angle class Ⅲ malocclusion using improved appliance FR Ⅲ.</p><p><b>METHODS</b>Forty patients with Angle class Ⅲ malocclusion were chosen and divided into two groups, namely, experimental and control. Each group had 20 patients. The young patients in the experi-mental group were treated using improved appliance FR Ⅲ, whereas those in the control group were treated using classic appliance FR Ⅲ. The hyoid bone position of the two groups were comparatively analyzed using an X-ray film before and after treatment.</p><p><b>RESULTS</b>Compared with the condition before treatment, the condition after treatment showed that the hyoid bone position of young patients with Angle class Ⅲ malocclusion treated using improved appliance FR Ⅲ, H-FH, H-S, H-Ptm, and Ar-H-Me exhibited an increased angle (P<0.01), whereas the hyoid bone position of those treated using H-MP and H-Gn showed a decreased angle (P<0.01). The hyoid bone position of young patients with Angle class Ⅲ malocclusion treated using classic appliance FR Ⅲ, H-FH, H-S, and H-Ptm had an increased angle (P<0.05). Moreover, the hyoid bone position of those treated using Ar-H-Me had an increased angle (P<0.01), and the hyoid bone position of those treated using H-MP and H-RGn had a decreased angle (P<0.05).</p><p><b>CONCLUSIONS</b>Compared with the hyoid bone position before treatment, the hyoid bone position after treatment of the young patients with Angle class Ⅲ malocclusion treated using improved appliance FR Ⅲ may move backward and downward, and the mandibular and hyoid bone position may move through clockwise rotation. The mandibular and hyoid bone position of young patients with Angle class Ⅲ malocclusion treated using classic appliance FR Ⅲ obtained a large angle by moving clockwise. The man-dibular bone moves backward and downward, thereby improving the hyoid bone in backward and upward directions. This condition makes a significant difference in treating the hyoid bone position of young patients with functional Angle class Ⅲ malocclusion. .</p>
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Humans , Hyoid Bone , Malocclusion, Angle Class III , MandibleABSTRACT
<p><b>OBJECTIVE</b>This study further explores the stromal cell-derived factor-1 (SDF-1)/chemokine receptor 4 (CXCR4) signaling axis mechanism in temporomandibular joint osteoarthritis (OA) by detecting the changes in CXCR4, interleukin (IL)-6, and collagen X expression in the ATDC5 cell line stimulated by the cyclic tensile strain and SDF-1.</p><p><b>METHODS</b>Insulin-transferrin-selenium (ITS) was used to induce ATDC5 cells to differentiate into chondrocyte-like cells. After three weeks, the cells were divided into two groups: those with and without cyclic tensile strain. These groups were further divided into the negative control and SDF-1 groups. Strain force of 20% was applied. After 12 h, the total proteins were extracted from cells of the four groups, and Western blot analysis was used to detect the changes in CXCR4, IL-6, and collagen X expression.</p><p><b>RESULTS</b>SDF-1 could enhance CXCR4, IL-6, and collagen X expressions in the chondrocytes, and 20% tensile strain force could further upregulate the three factors.</p><p><b>CONCLUSION</b>Under abnormal tensile force, SDF-1 can upregulate its specific receptor CXCR4, thus increasing its-binding efficiency and resulting in the activation of the SDF-1/CXCR4 axis. This condition enhances the expressions of IL-6 and other inflammatory factors and directly damages to cartilage tissue. Such damage directly promotes chondrocyte hypertrophy, which enhances collagen X expression.</p>
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Humans , Cell Differentiation , Cell Line , Chemokine CXCL12 , Collagen , Interleukin-6 , Receptors, CXCR4 , Signal Transduction , Stromal CellsABSTRACT
BACKGROUND:The regulatory role of extracellular signal regulated kinase 1/2 (ERK1/2) and nuclear factor kappa B (NF-κB) signal pathways in the osteogenic differentiation of MC3T3-E1 cells subjected to mechanical strain remains unclear. OBJECTIVE:To investigate the effects of ERK1/2 and NF-kB signal pathway on alkaline phosphatase, type Ⅰcol agen, osteocalcin and interleukin-6 expression in osteoblasts in response to mechanical strain, and to explore the regulatory effects of ERK1/2 and NF-kB signal pathway on osteoblast differentiation. METHODS:MC3T3-E1 cells cultured in vitro were separately treated with ERK1/2 pathway specific inhibitor PD098059 and NF-kB pathway inhibitor PDTC for 30 minutes, and subjected to12%elongation for 24 hours. Normal cells and cells along loading 12%mechanical strain for 24 hours were considered as controls. Enzyme linked immunosorbent assay and real-time PCR were utilized to detect alkaline phosphatase activities, type Ⅰcol agen, osteocalcin and interleukin-6 mRNA expression before and after cellloading. RESULTS AND CONCLUSION:Under 12%mechanical strain, alkaline phosphatase, type I col agen, and interleukin-6 expression was regulated by ERK1/2 signal pathway in MC3T3-E1 cells, but osteocalcin gene expression was not affected by ERK1/2 pathway. NF-kB signal pathway inhibitor PDTC significantly suppressed alkaline phosphatase activities in MC3T3-E1 cells under mechanical strain, and inhibited interleukin-6 gene expression. However, type I col agen and osteocalcin gene expression was not affected by NF-kB signal pathway. Results suggested that mechanical strain affected osteogenic differentiation and relevant gene expression in MC3T3-E1 cells by ERK1/2 and NF-kB signal pathway.
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Crossbite of upper and lower second molars is a common malocclusion. It is difficult to treat such cases. 4 kinds of treatments according to different situations are recommended. One is a mutual elastics on the upper and lower teeth. The second method is to move upper and lower teeth by archwire lingually and bucally. The third is to extract the second molars, while the third molars drift automatically. The fourth is using mini-implant as an anchorage to move the teeth to correct position. This article evaluated the advantages and disadvantages of the 4 kinds of methods. We also discussed how to choose the appliances as well as what we should pay attention to treat this malocclusion.
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OBJECTIVE: The purpose of this study was to evaluate the dentoskeletal and soft tissue profile changes after extraction of two lower first or second premolars in "borderline" adult skeletal Class III cases. METHODS: Twenty-eight patients with "borderline" skeletal Class III malocclusion were studied. All of them were treated by extraction of two lower first or second premolars. Lateral cephalometric radiographs taken at the start and end of treatment were analysed. Twenty-five cephalometric variables were calculated and paired t-tests were performed. RESULTS: After treatment, no significant changes were noted in the skeletal parameters (p > or = 0.05). Regarding the dental parameters, the L1-MP angle decreased by 8.1degrees, the U1-L1 angle increased by 7.7degrees (p < 0.01), the overjet distance increased by 5.7 mm (p < 0.01), the L1-NB angle decreased by 7.3degrees and the L1-NB distance decreased by 4.8 mm (p < 0.01). The soft tissue parameters of Li-E, Li-H and Li-RL2 distance decreased by 3.2 mm, 3.4 mm and 4.1 mm respectively (p < 0.01). CONCLUSIONS: Orthodontic camouflage treatment by extraction of two lower first or second premolars provides a viable treatment alternative for "borderline" skeletal Class III cases to achieve a good occlusal relationship.
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Adult , Humans , Bicuspid , Malocclusion , Tooth Movement TechniquesABSTRACT
<p><b>OBJECTIVE</b>To investigate the correlation between tongue size and openbite.</p><p><b>METHODS</b>The tongue size of the openbite patients and the subjects with normal occlusion was measured by B-type ultrasonic imaging respectively. The tongue size of the openbite patients and the subjects with normal occlusion was compared.</p><p><b>RESULTS</b>The tongue size in openbite group was larger than in normal occlusion group. The difference was statistically significant.</p><p><b>CONCLUSIONS</b>Tongue size was related to the openbite.</p>
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Adolescent , Adult , Child , Humans , Open Bite , Diagnostic Imaging , Pathology , Sensitivity and Specificity , Tongue , Diagnostic Imaging , Ultrasonography , MethodsABSTRACT
Objective To explore whether there is any abnormality in the tongue position of the open bite. Methods Tongue position was defined as the relative position of its body in the oral proper cavity on profile cephalometric roentgenogram which was enhanced by the iodine oil applied to the tongue surface. The tongue position was compared between open bite group and normal bite group with student's t test to determine the variation of open bite. Results Compared to the normal bite group, the root of tongue was closer to the uvula in the open bite group, and the distance between the dorsum of tongue and the surface of palate was decreased as well as the tip of tongue was nearer to the incisors. Conclusion The open bite's tongue position is characterized by more upward and more forward and this position may be one of the causes of open bite.
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Objective: To evaluate the effects of fixed inclined bite plate on jaw bone and dentition in juveniles with Class Ⅱ division 1 malocclusion.Methods: Eighteen patients(10 male and 8 female aged 11.5～13.8 years old) were treated with fixed inclined bite plate for bite-opening, twelve patients(6 male and 6 female) were involved as the control. Cephalograms were taken before and after the treatment.Results: After treatment ANB was significantly reduced and mandibular development was improved; AFH and PFH were significantly increased; the first molar in mandible was siginificantly elongated.Conclusion: Fixed inclined bite plate is effective in the improvement of sagittal skeletal relationship and in the orthodontic treatment in the juveniles with Class Ⅱ division 1 malocclusion.
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Implant anchorage depressed the elongated opposed teeth to prepare enough space for the mandibular dentition.Finally,a better dental implant prosthesis can be achieved.
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Objective:To compare shear bond strength(SBS)of bonded and rebonded orthodontic brackets and influence of rebonded orthodontic brackets using different treatment methods on SBS.Methods:60 extracted premolars were evenly divided into three groups.Brackets were debonded with conventional techniques and then removed of residual adhesives from the base mesh using three different treatments.In groupⅠ,rebond a new bracket at the second,third bonding times.In group Ⅱ,remove residual adhesives from the base mesh with fire at the second,third times,then rebond the same bracket.In group Ⅲ,remove that using a tungsten carbide bur at the second,third times,then rebond the same bracket.Shear test was performed to compare the SBS and adhesive remnant indexes(ARI)among different groups.Results:The SBS of three groups at the first bonding sequence were(10.00?2.95)MPa,(9.57?2.45)MPa and(9.09?2.58)MPa.The SBS at the second,third debonding sequence in group I,group II,and group III were(9.55?2.84)MPa and(10.32?2.59)MPa,(7.30?2.15)MPa and(7.14?1.93)MPa,(12.13?2.93)MPa and(12.86?3.08)MPa,respectively.In group Ⅱ,the SBS was significantly lower,however,in group Ⅲ,the SBS was significantly higher,and the results showed significant differences(P
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Objective: To evaluate the effects of the modified fixed r everse Twin-block appliance(TBA) combined with maxillary protraction appliance( MPA) in the treatment of early Angle Ⅲ skeletal anterior crossbite. Met hods:TBA combined with MPA was used in 15 growing subjects with early An gle Ⅲskeletal malocclusion (group TBA-MPA). Another 15 subjects with the same type of malocclusion were treated by MPA(group MPA). The effects of treatment we re studied by cephalometric measurments.Results:3-5 months afte r treatment SNB, B-Vert T, Po-Vert T,NPo-FH,Co-Po and 1-NB in group 1 were decreased more than those in group 2 (P0.05).Conclusion:Angle Ⅲ skeletal ante rior crossbite can be corrected successfully with reverse TBA combined with maxi llary protraction appliance by mandible retrusion and maxilla forward growth.
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Objective:To study the characters of perioral muscle p re ssure of the adult patients with skeletal crossbite. Methods:20 patients were diagnosed to be with skeletal crossbite. The perioral muscle press ures on upper and lower central incisors, first molars and cuspids were measured buccally and lingually at rest position. 10 health adults were served as the co ntrols. Results:The perioral force in skeletal crossbite group was larger than that in the control group on the correspondence area(P0.05). In the skeletal cross bite group, the perioral force on the mandibular area was larger than that on th e correspondence maxillary area(P0.05). The muscle pressure on the labial side was larger than that on t he lingual correspondence side in both groups(P
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0.05). Conclusion: The tongue measurement with ultrasonic imaging is reproducible.
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0.05). The pressure on the first molar from cheek was higher than that from tongue( P
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Objective: To investigate the effect of orthodontic and surgical treatments of skeletal crossbite on the electromyographic activity of human masticatory muscles. Methods: Electromyogram was used to test the changes of the electromyographic activity of masseter muscles, temporal anterior and diagastric anterior in 20 patients with skeletal crossbite before and after treatments. Results: Orthodontic and surgical treatments showed different effect on the activity of masseter muscle, temporal anterior and diagastric anterior. The change of the activity of the masseter muscle was the most significant among them. But, during chewing the masseter muscle was less active after treatments. Conclusion: Abnormal activity of the masticatory muscles can be partly corrected by the orthodontic and surgical treatments. The treatments cannot fully recover the function of the masseter muscle.
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Objective:To study the technique of treatment of ma lo cclusion with congenital individual missing lower incisor . Method s: Ten cases of malocclusion(male 3, femal 7) with congenital missi ng lower incisor were selected. The casts and cephalograms of pretreatment and posttreatment were measured and analysed respectively. The malocclusions were corrected by changing the position of jaws and teeth. Stripping, extraction and so on were applied in the treatment for the achievement of normal overjet and ov erbite according to the Bolton index. Results: Intercusp al bite, normal overjet and overbite were achieved in the ten cases. The majorit y of lower anterior teeth were anteversion. The width between lower canines decr eased. Conclusion: Satisfactory occlusion can be achieve d by orthodontic treatment in the patients of malocclusion with congenital indiv idual lower incisor missing.
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Objective: To evaluate the effect of a new method to treat early primary crossbite . Methods: 20 cases (2.8～6.5 years old ) with anterior crossbite were treated by bonding appliance combined with protracting mask. Cephalometric radiographs were taken and analyzed before and after treatment. Results: On average,in all the 20 cases the maxilla were moved 2.06 mm forward ,mandibular were turned 2.45?downward and backward ,the anterior crossbite was corrected and the face profile was improved satisfactorily.The treatment was conducted for 1.7 months on average. Conclusion: Using bonding appliance combined with protracting mask to treat early primary crossbite can achieve good effects for young children .
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0.05). Conclusion: The measurements of molar distal movement on model are smilar to those on cephalometric radiograph.
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objective: To evaluate the effect of Quad Helix appliance in expanding narrow dental arch. Methods: 12 cases were treated by Quad Helix appliance. The effect was evaluated by measurement of the dental arch. Results: The dental arch of all the patients was expanded in half a year.The width between the maxillary first molars increased by 8.95 mm, and the width between maxillary first premolars increased by 5.7 mm. The average time of expanding was 20.75 weeks, the expanding efficiency was 0.43 mm per week. Conclusion: Quad Helix is an effective orthodontic appliance for correction of narrow dental arch.